When comparing among various whey protein isolates (WPI) and lactoferrin at pH 3.5, 4.5, and 7.0, lactoferrin was astringent at pH 7.0 where no acid was added. CA200064-534 200064-534. The three pKa values for phosphoric acid (from the CRC Handbook of Chemistry and Physics ) are 2.16, 7… These acid constant values, along with the Henderson-Hasselbalch equation (section 7.2) tell us that, at the physiological \(pH\) of approximately 7, somewhat more than half of the phosphate species will be in the \(HPO_4^{-2}\) state, and slightly less than half will be in the \(H_2PO_4^{-1}\) state, meaning that the average net charge is between -1.5 and -2.0. Positive, because of the phosphate groups on the DNA backbone. Fortuitously, acetate fills this need well, with a buffering range of 3.8
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